The famous hospital is said to be the oldest hospital in the world. It occupies most of Tiberina Island. This Greek city was home to the celebrated temple of Asclepius, the god of medicine. According to the stories, Asclepius was with the mission of bringing back the healing serpent, believed to be the symbol of the god. Although there are several long gaps, the existence of a hospital on this island can be traced all the way through the Middle Ages. It was in 1588 that the Aqua Felice was first brought to the Tiberina Island.

The hospital and church of the Do Good for God Brothers were gifted an ounce of water, that is half an ounce free for the love of God and the other half against the usual payment. It is said that the friars rightly pointed out to the authorities that this water was mainly for the use of the sick that they piously assisted which on April 15, 1589 the City Authorities made a supporting decree. The Do Good Brothers later on managed to take things further and requested that they will be given a small donation and let them be granted twenty scudi in coin to bring the water to their Hospital for the use of the many poor sick and infirm they have there.

It was that time the Aqua Felice was taken to the ancient hospital with the source was the water returning from the Egyptian Lions Fountain spouts at the foot of the steps on the Capitoline Hill. Then a little fountain must then have been built in the hospital garden, one that would eventually become the fountain with the Boot of Italy on the bottom of its pool. Then the leafy garden was transformed into today's modest and insignificant cloister and the fountain also took on a completely new appearance as part of the radical reconstruction of the whole enormous building in 1934 by Cesare Bazzani. The fountain became the harmonious and pleasing arrangement we see today.